Protein precipitation

It also introduces the principles and applications of variety of sample preparation methods including solid phase extraction , protein precipitation , ultra-filtered centrifugation and affinity chromatography .

结合近年发表的文献具体阐述了固相萃取法、蛋白质沉淀法、超滤离心法、亲和层析法和部分其他方法的原理及其应用实例。

After a series of physical-chemical treatment which included protein precipitation , heating , centrifugation , decolorization , filtration and hydrolysis , the waste water was further treated by using biological enzyme Glucose Oxidase .

在经过蛋白质沉淀、加热、离心、脱色、过滤和水解等物理-化学法处理之后利用葡萄糖氧化酶将马铃薯淀粉废水进行生物酶降解反应。

METHOD S : The method involved protein precipitation with methanol .

方法血浆样品经甲醇沉淀后进行分析。

METHOD S : Plasma samples were treated by protein precipitation .

方法：样品预处理采用乙腈蛋白沉淀法。

Content determination of docetaxel in rat plasma by protein precipitation and HPLC method

蛋白沉淀-HPLC法测定大鼠血浆中多西他赛的含量

The plasma sample purification was limited to protein precipitation with acetonitrile .

血样加乙腈沉淀蛋白。

METHODS Plasma samples were treated by addition of ropivacaine as internal standard and protein precipitation before injection .

方法以甲磺酸罗哌卡因为内标，血浆样品经蛋白沉淀处理后，在0.2mL。

The analytes were extracted by protein precipitation , using chlorzoxazone as internal standard .

以氯唑沙宗为内标，血浆样品以蛋白沉淀法处理。

The sample treatment for propofol involved a simple protein precipitation with methanol , and thymol worked as internal standard .

以麝香草酚为内标，血清样品用甲醇沉淀蛋白后取上清液进样分析。

METHODS : Using naproxen as internal standard , mycophenolic acid in plasma samples was determined by HPLC with protein precipitation .

方法：以萘普生为内标，血浆用甲醇蛋白沉淀后直接进样。

OBJECTIVE To establish a ion pair RP HPLC method for the determination of gemcitabine in human plasma METHODS Samples were prepared by protein precipitation .

目的建立使用离子对反相高效液相色谱测定吉西他滨血药浓度的方法。

Astringency : The drug astringency function on the bowel mucosa and skin was observed with the method of exosomatic protein precipitation .

收敛：以体外蛋白沉淀法，对离体肠粘膜、皮肤的直接作用观察药物的收敛作用。

Cheese is the solid clots that through the enzyme reaction , casein protein precipitation , and then discharges the whey from the curd .

干酪是通过酶反应和酪蛋白沉淀，然后把乳清从凝乳中排出而得到的固体凝块。

The machanism of the formation of the protein precipitation in beer , the stability of the protein , the type and the property of the silicagel and its use in the stability of beer were stated .

介绍了啤酒中蛋白质沉淀形成的机理，蛋白质稳定技巧，硅胶的种类、特性及其在啤酒稳定方面的应用。

A protein precipitation method using 2 times of acetonitrile was developed to treat the samples of plasma and biological tissues , and the pharmacokinetics of Z-ligustilide in rabbits and tissue distribution in mice were investigated by intravenous injection .

建立了2倍体积的乙腈蛋白沉淀法处理血浆和生物组织样品，对当归、川芎挥发油微乳在家兔体内的药物动力学和小鼠体内组织分布进行了考察，方法学考察符合体内药物分析的要求。

( 7 ) The sample pretreatment is the key step in the biological sample analysis by HPLC . The sample pretreatment contains the protein precipitation and the analyte extraction , thus the operation is relatively laborious and time-consuming .

样品制备在HPLC的生物样品分析中是关键性的一步，经典的样品预处理包括蛋白质沉淀和药物萃取。

Methods : We purified the BSP protein by precipitation and affinity chromatography .

方法：利用盐析及亲和层析等方法对BSP蛋白进行纯化。

Collecting recombinant bacterias and ultrasonic crushing centrifugal , we found that the purpose protein in precipitation . It illustrated that he purpose protein was expressed in form of IB with no activity , needing renaturation to separation and purification .

离心收集重组体菌体，超声波破碎离心，发现沉淀中含有目的蛋白，说明目的蛋白以包含体的形式表达，没有活性，需要复性才能分离纯化。

The results showed that non-biological stabilization of rice wine was affected by mass presence of polymer protein , resulting in precipitation ;

结果表明：高分子蛋白质在酒中的大量存在必然影响黄酒非生物稳定性，导致浑浊沉淀；